Wilshire Technologies

Wilshire Technologies offers a full line of GLP and cGMP peptides for clinical and cosmetic research in quantities ranging from milligram to commercial scales.

A Certificate of Analysis, HPLC, and Mass Spec data accompany each peptide order, confirming both purity and identity. State-of-the-art equipment, fully validated processes and raw materials ensure the highest quality peptide synthesis. Years of peptide production experience allow us to continually improve our manufacturing efficiency with the resulting cost savings passed on to our customers.

Custom Peptide Syntheses

Applications for Custom Peptides include:

  • Biological effect measurements
  • Receptor characterization
  • Receptor-ligand interactions
  • Mass spec standard development and studies
  • Protein function-structure analysis
  • Antibody production (Polyclonal and Monoclonal)
  • Protease assays
  • Epitope mapping
  • ELISA standards

Custom Peptides Variables:

  • Sequence lengths: 2 - 60 amino acids
  • Fast turn-around: Typically a crude product -2-3 weeks; purified in 3-4 weeks
  • TFA, HCI, or Acetate salt (counter-ion) options
  • cGMP/GLP grades available
  • mg to multi-Kg quantities
  • A range of deliverable purities.
Crude to 80% - lead generation; polyclonal antibodies; high-throughput screening
> 80% - tissue culture; ligand for affinity purification; antibody blocking experiments
> 90% - in vivo studies; bioassays; markers for electrophoresis; monoclonal antibodies
> 95% - ELISA; RIA; enzyme substrate; in vitro studies
> 98% - NMR; chromatography standards

Each custom peptide synthesis order is supplied lyophilized with:

  • CoA (representative)
  • MALDI-TOF Mass Spec to confirm identity (representative)
  • Amino Acid Analysis (AAA) available upon request
  • Reverse-phase HPLC to confirm purity (representative)
  • Peptide content by Nitrogen analysis

Custom Peptide Modifications

WTI offers a full line of custom peptide modifications, including an extensive selection of non-natural amino acids, modified side chains, phosphorylation, conjugations and both N- and C-terminal modifications.

Dye labeled peptides – full range of dyes are available: yellow, green, purple, red, etc.

Fluorescent peptides – important in the study of protein-protein interactions conformational analyses, substrates design, etc.

Phosphorylated Peptides – Ser, Thr, and Tyr (single site) phosphorylation

Biotin-labeled Peptides – Biotinylation - with or without a spacer (Ahx) - via either the sulfhydryl (SH) group or amino (NH2) group.

Fatty Acid Labeled Peptides – Palmitoyl, myristoyl, capryl, octyl, etc.

Disulfide-containing Peptides - Important structure motifs, achieved by the oxidation of thiol groups to form disulfide bonds

Citrulline-containing Peptides

Most Common N-Terminal Modifications

  • Acetylation
  • Acetylation (Lys)
  • Formylation
  • Fatty Acid Amidation
    • Myristic Acid
    • Palmitoyl/Palmitic Acid
  • Cys(Acm), Cys(tBu)
  • Benzyloxycarbonylation (CBZ)

Most Common C-Terminal Modifications

  • Amidation
  • p-Nitroanilide (pNA)
  • AMC
  • NHS (OSu)
  • CMK/FMK
  • Succinylation (Suc)
  • Prenylation (Geranylgeranidation)

Incorporation of the Geranylgeranyl (C20) isoprenoid moiety onto the C-terminus of many proteins results in a natural modification that permits their direct penetration into the plasma membrane of the cell.

These modified peptides are able to efficiently cross the cell membrane in an ATP-independent, nonendocytic manner. Studies have revealed that the sequence of the peptide does not affect uptake [Wollack, J. W., et al., 2009].

Further studies have shown that the prenyl group is the major determinant in allowing these peptides to enter cells. The presence of a hydrophobic fluorophore has little effect.

Additional services such as peptide content reporting, aliquoting, salt removal and specific counter-ion requirements (HCl, Acetate, TFA) are also available.

cGMP Facility

In 2012, our 60,000 sq. ft., state-ol-the-art, US FDA registered cGMP facility was inaugurated.

  • Eight independent production suites.
  • Fully automated synthesizers from S-L to 100-L.
  • Custom and standard COA packages.
  • Single-batch scale capability of up to S Kg of purified peptide.
  • Instrumentation that meets or exceeds industry standards.

GMP Quality System

GMP peptide manufacturing is performed under the protocols set forth in FDA 21 CFR parts 210 & 211, following ICH Q7A Good Manufacturing Practices. These dedicated facilities include solid-phase reactors, purification systems, lyophilization and packaging. [When applications don't require a cGMP-grade peptide, e.g. animal tox studies, GLP services are also available.] Class 300,000/100,000/10,000 clean rooms, and on-site water treatment facilities round-out the comprehensive GMP Peptide and API manufacturing services. Site visits and audits welcomed.